Journal:

Article Title: Fibrosis in left atrial tissue of patients with atrial fibrillation with and without underlying mitral valve disease

doi: 10.1136/hrt.2003.015347

Figure Lengend Snippet: Sirius red staining of left atrial tissue for connective tissue (red staining) from patients (A) in sinus rhythm (SR) and (B) with chronic atrial fibrillation (lone CAF). Immunohistological results in left atrial tissue from (left) patients in SR and (right) patients with lone CAF for (C, D) collagen type I, (E, F) collagen type III, and (G, H) fibronectin. Original magnification ×400.

Article Snippet: Goat anti-human collagen I, goat anti-human collagen III (Santa Cruz Biotechnology) and rabbit anti-human fibronectin (DPC Biermann, Bad Nauheim, Germany) were used as primary antibodies.

Techniques: Staining

Journal:

Article Title: Fibrosis in left atrial tissue of patients with atrial fibrillation with and without underlying mitral valve disease

doi: 10.1136/hrt.2003.015347

Figure Lengend Snippet: (A) Collagen I, collagen III, and fibronectin expression in atrial tissue of (left columns) patients in SR (left columns) and (right columns) patients with atrial fibrillation. (B) Comparison of all patients in the study either (left columns) with underlying mitral valve disease (MVD) or (right columns) without MVD.

Article Snippet: Goat anti-human collagen I, goat anti-human collagen III (Santa Cruz Biotechnology) and rabbit anti-human fibronectin (DPC Biermann, Bad Nauheim, Germany) were used as primary antibodies.

Techniques: Expressing, Comparison

Journal:

Article Title: Fibrosis in left atrial tissue of patients with atrial fibrillation with and without underlying mitral valve disease

doi: 10.1136/hrt.2003.015347

Figure Lengend Snippet: (A) Collagen I, (B) collagen III, and (C) fibronectin expression in left atrial tissue of patients in SR, with lone AF, and with AF with underlying MVD (MVD+AF).

Article Snippet: Goat anti-human collagen I, goat anti-human collagen III (Santa Cruz Biotechnology) and rabbit anti-human fibronectin (DPC Biermann, Bad Nauheim, Germany) were used as primary antibodies.

Techniques: Expressing

Journal:

Article Title: Fibrosis in left atrial tissue of patients with atrial fibrillation with and without underlying mitral valve disease

doi: 10.1136/hrt.2003.015347

Figure Lengend Snippet: Quantitative analysis of (A) collagen I, (B) collagen III, and (C) fibronectin expression in human atrial tissue of the SR, paroxysmal atrial fibrillation (PAF), and CAF subgroups of lone AF and MVD+AF.

Article Snippet: Goat anti-human collagen I, goat anti-human collagen III (Santa Cruz Biotechnology) and rabbit anti-human fibronectin (DPC Biermann, Bad Nauheim, Germany) were used as primary antibodies.

Techniques: Expressing

Journal: Molecular Vision

Article Title: Downregulation of LIM kinase 1 suppresses ocular inflammation and fibrosis

doi:

Figure Lengend Snippet: LIMK1 siRNA downregulates LIMK1 protein and suppresses fibronectin deposition in human corneal fibroblasts. A : western blot analysis. Corneal fibroblasts were transfected as indicated for 72 h. Cells were lysed and protein levels were analyzed using LIMK1 and GAPGH antibodies. B-E : Immunofluorescence microscopy using fibronectin antibody and DAPI staining in fibroblast cultures ( B ) untreated; ( C ) treated with 100 nM scrambled control siRNA; ( D ) treated with 50 nM of LIMK1 -targeted siRNA; or ( E ) treated with 100 nM of LIMK1 -targeted siRNA. F : Quantitative analysis of fibronectin secreted to the culture media by western blotting. Samples were untreated or treated with control or LIMK1 -targeted siRNA as indicated. Experiments were performed 3 times yielding similar results. Error bars represent standard deviation. The asterisk indicates a p<0.025 (n=3) compared to scrambled siRNA.

Article Snippet: To carry out immunostaining, corneal fibroblasts were incubated with polyclonal rabbit anti-human fibronectin (1:100; BD Biosciences, Lexington, KY) or monoclonal anti-paxillin (1:100; Millipore, Billercia, MA).

Techniques: Western Blot, Transfection, Immunofluorescence, Microscopy, Staining, Standard Deviation